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The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 2: Functional Studies provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 1: Library Construction, Physical Mapping, and Sequencing, presents readily reproducible techniques for library construction, physical mapping, and sequencing. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.
Bacterial artificial chromosomes -- Laboratory manuals. --- Bacterial chromosomes. --- Electronic books. -- local. --- Biology --- Health & Biological Sciences --- Cytology --- Bacterial artificial chromosomes --- Chromosomes --- BACs (Chromosomes) --- Artificial chromosomes --- Bacterial chromosomes --- Microbiology. --- Microbial biology --- Microorganisms --- Chromosome mapping --- Chromosomes, artificial, bacterial --- Cloning, molecular --- Genetic structures --- Genomic library
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bibliographies --- chromosomes --- evolution --- genetic variation --- hybridization --- polyploidy --- rare species --- speciation
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The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.
Bacterial artificial chromosomes -- Laboratory manuals. --- Bacterial chromosomes. --- Electronic books. -- local. --- Biology --- Cytology --- Health & Biological Sciences --- Bacterial artificial chromosomes --- Gene Library --- Genetic Techniques --- Chromosomes, Artificial --- Investigative Techniques --- Chromosomes --- Genetic Structures --- Chromosome Mapping --- Chromosomes, Artificial, Bacterial --- Cloning, Molecular --- Genomic Library --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cellular Structures --- Genetic Phenomena --- Phenomena and Processes --- Cells --- Anatomy --- BACs (Chromosomes) --- Artificial chromosomes --- Bacterial chromosomes --- Bacteriology. --- Microbiology. --- Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Microbial biology --- Microorganisms --- Microbiology --- Composition --- Chromosome mapping --- Chromosomes, artificial, bacterial --- Cloning, molecular --- Genetic structures --- Genomic library
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Gene expression. --- Genetic Techniques. --- Genetics, Population. --- Chromosomes, human --- Genes --- Genome, human --- Human --- Human genome project
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Genes --- Chromosomes, Human --- Molecular Biology --- Genetics, Medical --- Preventive Medicine --- Genetic Testing --- Human chromosomes --- Molecular genetics --- Medical genetics --- Medicine, Preventive --- Genetic screening --- Gènes --- Chromosomes humains --- Génétique moléculaire --- Génétique médicale --- Médecine préventive --- Dépistage génétique --- Genetica --- Génétique --- Gènes --- Génétique moléculaire --- Génétique médicale --- Médecine préventive --- Dépistage génétique --- Genetics, Medical. --- Genes. --- Chromosomes, Human. --- Molecular Biology. --- Preventive Medicine. --- Genetic Testing.
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The Institute for Mathematical Sciences at the National University of Singapore organized a program on "Post-Genome Knowledge Discovery" from January to June 2002. The program focused on the computational and statistical analysis of sequences and genetics, and the mathematical modeling of complex biological interactions, which are critical to the accurate annotation of genomic sequences, the study of the interplay between genes and proteins, and the study of the genetic variability of species. As part of the program, tutorials for graduate students and newcomers to this transdisciplinary area
Genetics --- Genetics. --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Mathematics. --- Information systems
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Genetics --- Génétique --- Genetics. --- Genetic Structures --- Genetic Phenomena --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology)
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Genetics, Microbial. --- Chromosomes, Bacterial --- Microorganisms --- Micro-organismes --- genetics. --- Evolution. --- Evolution --- 579.25 --- 579.26 --- Microbial genetics --- Microbial ecology --- 579.26 Microbial ecology --- 579.25 Microbial genetics --- Genetics. --- Microbial evolution --- Evolution (Biology)
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Genetic recombination plays an essential role in such vitally important biological processes as DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. In Genetic Recombination: Reviews and Protocols, prominent international investigators actively engaged in recombination research describe in great detail their best techniques for studying recombination. The methods range from approaches and model systems to be used in a variety of eukaryotic organisms and in a mammalian parasite, to biochemical analyses useful for furthering the understanding of recombination mechanisms. Additional techniques use recombination as a reporter of genomic instability in lower and higher eukaryotes and as a tool for producing targeted genetic modification. Each readily reproducible method includes step-by-step instructions, a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Genetic Recombination: Reviews and Protocols offers both novice and experienced investigators an illuminating synthesis of what we currently know about recombination, as well as a wide-ranging collection of powerful tools for elucidating its mechanisms and regulation throughout biology today.
Gene Expression. --- Gene Targeting. --- Genetic Engineering. --- Genetic recombination. --- Recombination, Genetic. --- Gene expression. --- Genes --- Recombination, Genetic --- Expression --- Genetic regulation --- Chromosomes --- Recombinant DNA --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists
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575 --- 575 General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- General genetics. General cytogenetics. Immunogenetics. Evolution. Speciation. Phylogeny --- Genetics. --- Genetics --- Biology --- Embryology --- Mendel's law --- Adaptation (Biology) --- Breeding --- Chromosomes --- Heredity --- Mutation (Biology) --- Variation (Biology) --- Molecular biology
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